http://hdl.handle.net/1807/3323 Mon, 10 Jul 2017 07:46:19 GMT 2017-07-10T07:46:19Z http://hdl.handle.net/1807/64946 Title: Taxonomical notes on selected freshwater fish species described from northern and central Vietnam (Cypriniformes: Balitoridae, Cobitidae, Cyprinidae, Nemacheilidae; Perciformes: Channidae, Osphronemidae; Synbranchiformes: Mastacembelidae) Author: Endruwei, Marco Description: Selected, little known taxa of northern and central Vietnamese freshwater fish species are reviewed. Nomenclatural acts are taken: Hemibarbus lehoai is placed in synonymy of H. maculatus , Paracobitis hagiangensis in synonymy of Schistura caudofurca . A neotype of Micronemacheilus bacmeensis is assigned. The name Channa hanamensis is treated as a nomen nudum. Two labeonine species described from China are nomenclaturally affected: Garra findolabium is transferred to Vinagarra and its specific epithet is treated as a noun in apposition; the specific epithet of Sinigarra napoense is corrected to napoensis. Mon, 31 Mar 2014 00:00:00 GMT http://hdl.handle.net/1807/64946 2014-03-31T00:00:00Z http://hdl.handle.net/1807/64942 Title: Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream ( Megalobrama amblycephala ) Author: TANG, Shou-Jie; LI, Si-Fa; CAI, Wan-Qi; ZHAO, Yan Description: In the present study, the genetic diversity of one selected strain (Pujiang No. 1), two domesticated populations (GA and HX) and four wild populations (LZ, YN, SS and JL) of blunt snout bream ( Megalobrama amblycephala ) was analyzed using 17 microsatellite markers. The results showed that an average of 4.88-7.65 number of alleles (A); an average of 3.20−5.33 effective alleles (Ne); average observed heterozygosity (Ho) of 0.6985−0.9044; average expected heterozygosity (He) of 0.6501−0.7805; and the average polymorphism information content (PIC) at 0.5706−0.7226. Pairwise FST value between populations ranged from 0.0307−0.1451, and Nei’s standard genetic distance between populations was 0.0938−0.4524. The expected heterozygosities in the domesticated populations (GA and HX) were significantly lower than those found in three wild populations (LZ, SS and JL), but no difference was detected when compared with the wild YN population. Likewise, no difference was found between the four wild populations or two domesticated populations. The expected heterozygosity in Pujiang No. 1 was higher than the two domesticated populations and lower than the four wild populations. Regarding pairwise FST value between populations, permutation test P-values were significant between the GA, HX and PJ populations, but not between the four wild populations. These results showed that the expected heterozygosity in the selected strain of blunt snout bream, after seven generations of selective breeding, was lower than that of wild populations, but this strain retains higher levels of genetic diversity than domesticated populations. The genetic differences and differentiation amongst wild populations, domesticated populations and the genetically improved strain of blunt snout bream will provide important conservation criteria and guide the utilization of germplasm resources. Mon, 31 Mar 2014 00:00:00 GMT http://hdl.handle.net/1807/64942 2014-03-31T00:00:00Z http://hdl.handle.net/1807/64944 Title: Effects of hydroperiod duration on developmental plasticity in tiger frog ( Hoplobatrachus chinensis ) tadpoles Author: FAN, Xiao-Li; LIN, Zhi-Hua; WEI, Jie Description: While developmental plasticity can facilitate evolutionary diversification of organisms, the effects of water levels as an environmental pressure on tiger frogs remains unclear. This study clarifies the relationship by studying the responses of tiger frog ( Hoplobatrachus chinensis ) tadpoles to simulated hydroperiods (i.e., constant low water levels, constant high water levels, increasing water levels, decreasing water levels, rapid changes in water levels and gradual fluctuations in water levels) in a laboratory setting. ANOVA analysis showed that none of the water level treatments had any significant effect on the total length, body mass, or developmental stages of H. chinensis tadpoles half way through development (11 days old). Tadpoles raised in rapidly fluctuating water levels had protracted metamorphosis, whereas tadpoles raised under low and gradually fluctuating water levels had shortened metamorphosis. None of the water level treatments had a significant effect on the snout-vent length (SVL) or body mass of H. chinensis tadpoles at Gosner stage 42, or on the body mass of tadpoles at Gosner stage 45. However, the tadpoles raised in high levels and rapidly fluctuating water levels, significantly larger SVL at Gosner stage 45, while ones under gradually fluctuating water levels had smaller SVL than the other groups. Time to metamorphosis was positively correlated with body size (SVL) at metamorphosis in H. chinensis tadpoles. H. chinensis tadpoles under constant low water level had the highest mortality rate among all the treatments (G-test). Moreover, ANOVA and ACNOVA (with body length as the covariate) indicated that water levels had no significant effect on either the morphology (i.e. head length, head width, forelimb length, hindlimb length and body width) or the jumping ability of juvenile H. chinensis. These results suggest that the observed accelerated metamorphosis and high mortality of H. chinensis tadpoles under decreasing water level treatment was driven by density-induced physical interactions among increasing conspecifics. Mon, 31 Mar 2014 00:00:00 GMT http://hdl.handle.net/1807/64944 2014-03-31T00:00:00Z http://hdl.handle.net/1807/64943 Title: Effects of cold narcosis on memory acquisition, consolidation and retrieval in honeybees ( Apis mellifera ) Author: CHEN, Yan-Mei; FU, Yu; HE, Jing; WANG, Jian-Hong Description: In learning and memory studies on honeybees ( Apis mellifera ), cold-induced narcosis has been widely used to temporarily immobilize honeybees. In this study, we investigated the effects of cold narcosis on the associative memories in honeybees by using the proboscis extension response (PER) paradigm. Severe impairments in memory acquisition was found when cold narcosis was performed 30 min, instead of 1 h before training. Locomotor activities were reduced when honeybees were tested 15 min, instead of 30 min after cold narcosis. These results indicate that cold narcosis impairs locomotor activities, as well as memory acquisition in a time-dependent manner, but by comparison no such effects on memory retrieval have yet been observed.[0] Mon, 31 Mar 2014 00:00:00 GMT http://hdl.handle.net/1807/64943 2014-03-31T00:00:00Z