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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/10443

Title: A Bilevel Optimization Algorithm to Identify Enzymatic Capacity Constraints in Metabolic Networks - Development and Application
Authors: Yang, Laurence
Advisor: Cluett, William R.
Mahadevan, Radhakrishnan
Department: Chemical Engineering and Applied Chemistry
Keywords: constraint-based modeling
metabolic engineering
flux balance analysis
redox
Issue Date: 25-Jul-2008
Abstract: Constraint-based models of metabolism seldom incorporate capacity constraints on intracellular fluxes due to the lack of experimental data. This can sometimes lead to inaccurate growth phenotype predictions. Meanwhile, other forms of data such as fitness profiling data from growth competition experiments have been demonstrated to contain valuable information for elucidating key aspects of the underlying metabolic network. Hence, the optimal capacity constraint identification (OCCI) algorithm is developed to reconcile constraint-based models of metabolism with fitness profiling data by identifying a set of flux capacity constraints that optimally fits a wide array of strains. OCCI is able to identify capacity constraints with considerable accuracy by matching 1,155 in silico-generated growth rates using a simplified model of Escherichia coli central carbon metabolism. Capacity constraints identified using experimental fitness profiles with OCCI generated novel hypotheses, while integrating thermodynamics-based metabolic flux analysis allowed prediction of metabolite concentrations.
URI: http://hdl.handle.net/1807/10443
Appears in Collections:Master
Department of Chemical Engineering and Applied Chemistry - Master theses

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