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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/1158

Title: RESEARCH ARTICLE - Novel technique for microbial production of 3,4-Dihydroxy Phenyl Lalanine by a mutant strain of Aspergillus oryzae
Authors: Ali, Sikander
Qadeer, M. A.
Issue Date: Aug-2002
Publisher: Universidad Católica de Valparaíso -- Chile
Citation: Electronic Journal of Biotechnology 5(2)
Abstract: L-DOPA is a useful drug for Parkinson’s disease. This investigation deals with the biosynthesis of L-DOPA by parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae. There was a marked difference between the mycelial morphology and pellet type of the parental and UV-irradiated mutant cultures. The mutant strain of Aspergillus oryzae UV-6 showed pellet-like mycelial morphology and improved tyrosinase activity. Mould mycelium was used for biochemical conversion of L-tyrosine to L-DOPA since tyrosinase is an intracellular enzyme. The mutant was found to give 3.72 folds higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed greater ability of the mutant to yield L-DOPA (i.e. Yp/x 32.73 mg/mg with parent and 95.71 mg/mg with mutant). When cultures grown for various incubation periods, were monitored for Qp, Qs and qp, there was significant enhancement (p<0.0025-0.005) in these variables by the mutant strain of Aspergillus oryzae UV-7 over GCB-6 on all the rates.
URI: http://bioline.utsc.utoronto.ca/archive/00000286/01/ej02022.pdf
Appears in Collections:Bioline International Legacy Collection

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