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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/16718

Title: Translational Regulation in the Early Drosophila Embryo
Authors: Nelson, Meryl
Advisor: Smibert, Craig
Department: Biochemistry
Keywords: Drosophila
Translational regulation
Issue Date: 19-Jan-2009
Abstract: Translational regulation is an important mechanism for regulating gene expression. Regulation of specific transcripts is often mediated by elements present in the 3′ untranslated region (UTR) of the mRNA. In the Drosophila embryo, translational repression of nanos mRNA is mediated by Smaug protein bound to specific sequences present in the 3′ UTR of the mRNA. Here I show that Smaug recruits Cup protein to the mRNA and that Cup in turn binds to the translation initiation factor eIF4E that is present at the 5′. The interaction between Cup and eIF4E prevents formation of a productive translation initiation complex on the mRNA. Therefore, Smaug-dependent translational repression functions at the initiation step via the indirect interaction of Smaug with eIF4E, which is mediated by Cup. In the second example of translational regulation presented here, I show that an element present in the 3′ untranslated region of the Hsp83 mRNA mediates translational enhancement in the Drosophila embryo. I have identified three proteins, Hrp48, DDP1 and PABP that interact with this element and have demonstrated that Hrp48 and DDP1 function in translational enhancement.
URI: http://hdl.handle.net/1807/16718
Appears in Collections:Doctoral
Department of Biochemistry - Doctoral theses

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