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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/16722

Title: Integration of Micro and Nanotechnologies for Multiplexed High-Throughput Infectious Disease Detection
Authors: Klostranec, Jesse
Advisor: Chan, Warren C. W.
Department: Biomedical Engineering
Keywords: Nanotechnology
Infectious Disease Diagnostics
Multiplexed Detection
Issue Date: 19-Jan-2009
Abstract: This thesis presents the development and optimization of a high-throughput fluorescence microbead based approach for multiplexed, large scale medical diagnostics of biological fluids. Specifically, different sizes of semiconductor nanocrystals, called quantum dots, are infused into polystyrene microspheres, yielding a set of spectrally unique optical barcodes. The surface of these barcodes are then used for sandwich assays with target molecules and fluorophore-conjugated detection antibodies, changing the optical spectra of beads that have associated with (or captured) biomolecular targets. These assayed microbeads are analyzed at a single bead level in a high-throughput manner using an electrokinetic microfluidic system and laser induced fluorescence. Optical signals collected by solid state photodetectors are then processed using novel signal processing algorithms. This document will discuss developments made in each area of the platform as well as optimization of the platform for improved future performance.
URI: http://hdl.handle.net/1807/16722
Appears in Collections:Doctoral
Institute of Biomaterials and Biomedical Engineering - Doctoral theses

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