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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17184

Title: Searching for Radiosensitizers: Development of a Novel Assay and High-throughput Screening
Authors: Katz, David
Advisor: Liu, Fei-Fei
Department: Medical Biophysics
Keywords: Radiosensitizer
High-Throughput Screening
Colony Formation Assay
Issue Date: 24-Feb-2009
Abstract: The colony formation assay (CFA) is the gold standard for measuring cytotoxic effects on cells. To increase efficiency, the CFA was converted to a 96-well format using an automated colony counting algorithm. The 96-well CFA was validated using ionizing radiation (IR) on the FaDu and A549 cancer cell lines. Its ability to evaluate combination therapies was investigated using cisplatin and IR. The 96-well CFA was transferred to a robotic platform for evaluation as a high-throughput screen (HTS) readout for the discovery of novel anti-cancer compounds, and radiosensitizers. Screening yielded eight putative anti-cancer hits, and five putative radiosensitizing hits. Secondary screening confirmed 6/8 anti-cancer compounds, and 0/5 radiosensitizing compounds. Thus, the 96-well CFA can be adopted as an alternative assay to the 6-well CFA in the evaluation of cytotoxicity in vitro, providing a possible readout to be utilized in HTS for discovering anti-cancer compounds, but with limited applicability in discovering radiosensitizers.
URI: http://hdl.handle.net/1807/17184
Appears in Collections:Master
Department of Medical Biophysics - Master theses

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