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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17186

Title: Defining a Model of Classical Activation in Microglia
Authors: Kena-Cohen, Veronique
Advisor: Schlichter, Lyanne C.
Department: Physiology
Keywords: Neuroinflammation
Microglia
Cytokine
cAMP Response Element Binding Protein
Issue Date: 24-Feb-2009
Abstract: Microglia, the resident immune cells of the central nervous system, can become activated following injury, disease, or infection. In vitro, they can be activated by stimuli, which determine the inflammatory phenotype they will develop. In this thesis, stimulating microglia with tumor necrosis factor- and interferon- resulted in classical activation, characterized by proliferation, increased transcription of complement receptor 3 and major histocompatibility class II molecules, and elevated production and transcription of interleukin-1 and nitric oxide. Stimulation with TNF and IFN also changed the intensity of phosphorylated (activated) cyclic adenosine monophosphate response element binding protein immunoreactivity in microglia. Specifically, cells differentiated into populations with high or low pCREB intensity. This was the first example of such a response in microglia and was representative of what occurred in vivo, after ICH. Thus, the characterization of this model will be useful for future studies of this and other intracellular pathways of classically activated microglia.
URI: http://hdl.handle.net/1807/17186
Appears in Collections:Master
Department of Physiology - Master theses

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