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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17192

Title: Controlling Cell Density by Micropatterning Regulates Smad Signalling and Mesendoderm Differentiation of Human Embryonic Stem Cells
Authors: Lee, Lawrence
Advisor: Zandstra, Peter W.
Department: Biomedical Engineering
Keywords: Pluripotent Stem Cells
Mesendoderm
Hematopoiesis
Micropatterning
Issue Date: 24-Feb-2009
Abstract: Human embryonic stem cells (hESC) present a potentially unlimited supply of hematopoietic progenitors for cell-based therapies. However, current protocols for generating these progenitors typically also generate undesired cell types due to imprecise control of the hESC microenvironment and poor understanding of the signalling networks regulating mesoderm differentiation (the germ layer from which hematopoietic cells emerge). This report demonstrates that activation of the downstream effectors of Activin/Nodal and bone morphogenetic protein (BMP) signalling (Smad2 (composite of Sma (smaller) and Mad (mothers against decapentaplegic) and Smad1, respectively) are both required for mesoderm differentiation. It is further shown that microcontact printing-mediated control of hESC colony size creates local microenvironments that guide differentiation, via a Smad1-dependent mechanism, preferentially towards the mesoderm lineage. These findings demonstrate the need for precise control of the microenvironment in order to effectively guide hESC differentiation to produce specific cell types for potential therapeutic applications.
URI: http://hdl.handle.net/1807/17192
Appears in Collections:Master
Institute of Biomaterials and Biomedical Engineering - Master theses

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