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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17205

Title: Functional Consequences of Complete GSK-3 Ablation in Mouse Embryonic Fibroblasts
Authors: Miron, Ioana
Advisor: Woodgett, James
Department: Medical Biophysics
Keywords: GSK-3
Issue Date: 24-Feb-2009
Abstract: Glycogen Synthase Kinase-3 (GSK-3) is a highly conserved serine/threonine kinase comprised of two mammalian homologues, GSK-3α and β, encoded by independent genes. This thesis reports the characterization of GSK-3-null primary mouse embryonic fibroblasts (MEFs) generated by gene targeting to gain insight into the physiological functions of this protein kinase. Combined inactivation of both alleles of GSK-3α and GSK-β led to elevated sensitivity to TNFα-induced apoptosis, altered organization of focal adhesion complexes, defects in cell spreading on fibronectin, decreased cell growth associated with altered cell cycle progression through the G2/M phase and increased spontaneous apoptosis. Future work will focus on unraveling the molecular mechanisms responsible for these effects and identifying the common and distinct cellular roles for GSK-3α and β, and specific variants of these isoforms.
URI: http://hdl.handle.net/1807/17205
Appears in Collections:Master
Department of Medical Biophysics - Master theses

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