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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17206

Title: ALX4 Expression in the Normal Breast and in Breast Cancer
Authors: Mohabir, Nadia
Advisor: Hamel, Paul A.
Department: Laboratory Medicine and Pathobiology
Keywords: ALX4
mammary gland
breast cancer
Issue Date: 24-Feb-2009
Abstract: Aristaless-like homeobox 4 is a homeodomain transcription factor that has important functions during mouse development. A recent report demonstrated that Alx4 expression is required in periductal stromal cells in the mouse mammary gland for normal mammary morphogenesis. To test the hypothesis that ALX4 is expressed in the normal human breast, and this expression is altered in breast cancer, immunohistochemistry was performed on normal and breast cancer tissue and breast tissue microarrays. In the normal breast, ALX4 was expressed in stromal fibroblasts and luminal epithelial cells, but not in myoepithelial cells. Expression was lost in breast cancer in both cell compartments. Upon global demethylation induced by 5-aza-2’-deoxycytidine, normal and breast cancer cell lines expressed ALX4, suggesting that hypermethylation may repress expression of ALX4 during malignant transformation of the breast. These results demonstrate that ALX4 may be used as a biomarker for breast cancer, and may act as a tumour suppressor.
URI: http://hdl.handle.net/1807/17206
Appears in Collections:Master
Department of Laboratory Medicine and Pathobiology - Master theses

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