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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17217

Title: Utility of the Cytochrome Oxidase I gene (COI) for Species Identification and Phylogeographic Analysis in Black Flies (Diptera: Simuliidae)
Authors: Rivera Castillo, Julio Martin
Advisor: Currie, Douglas C.
Department: Ecology and Evolutionary Biology
Keywords: DNA Barcoding
Population Genetics
Issue Date: 26-Feb-2009
Abstract: A short sequence of ca. 658-bp of the mitochondrial gene COI was used to investigate its utility as a DNA barcode in the medically important Simuliidae or black flies. Sixty-five species and species complexes were tested. Results indicate that the barcoding gene discriminated among morphologically distinct species with nearly 100% of efficacy and proved useful for revealing cryptic diversity. The DNA barcoding gene was also tested for revealing phylogeographic patterns in the western cordilleran Prosimulium travisi and the Prosimulium neomacropyga species-group. Phylogeographic analyses on these species revealed areas that acted as glacial refugia, postglacial history, cryptic speciation episodes and timing of the events that lead to their present-day distribution. The results obtained concur with other phylogeographic studies on similarly-distributed cordilleran organisms. In conclusion, the barcoding gene not only resulted useful for species discrimination in black flies but also for studies at the population level, providing value-added to this molecular marker.
URI: http://hdl.handle.net/1807/17217
Appears in Collections:Master
Department of Ecology & Evolutionary Biology - Master theses

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