T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Please use this identifier to cite or link to this item:
|Title: ||Utility of the Cytochrome Oxidase I gene (COI) for Species Identification and Phylogeographic Analysis in Black Flies (Diptera: Simuliidae)|
|Authors: ||Rivera Castillo, Julio Martin|
|Advisor: ||Currie, Douglas C.|
|Department: ||Ecology and Evolutionary Biology|
|Keywords: ||DNA Barcoding|
|Issue Date: ||26-Feb-2009|
|Abstract: ||A short sequence of ca. 658-bp of the mitochondrial gene COI was used to investigate its utility as a DNA barcode in the medically important Simuliidae or black flies. Sixty-five species and species complexes were tested. Results indicate that the barcoding gene discriminated among morphologically distinct species with nearly 100% of efficacy and proved useful for revealing cryptic diversity. The DNA barcoding gene was also tested for revealing phylogeographic patterns in the western cordilleran Prosimulium travisi and the Prosimulium neomacropyga species-group. Phylogeographic analyses on these species revealed areas that acted as glacial refugia, postglacial history, cryptic speciation episodes and timing of the events that lead to their present-day distribution. The results obtained concur with other phylogeographic studies on similarly-distributed cordilleran organisms.
In conclusion, the barcoding gene not only resulted useful for species discrimination in black flies but also for studies at the population level, providing value-added to this molecular marker.|
|Appears in Collections:||Master|
Department of Ecology & Evolutionary Biology - Master theses
Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.