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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17220

Title: Analyses of Host Specificity, Immune Interactions and New Virulence Candidates of Pseudomonas syringae
Authors: Sanina, Natali
Advisor: Guttman, David S.
Department: Cell and Systems Biology
Keywords: host specificity
Pseudomonas syringae
immune interactions
Issue Date: 26-Feb-2009
Abstract: We studied the host specificity, interactions with plant immune systems, and virulence factors of the phytopathogenic Type III secretion system-carrying bacterium Pseudomonas syringae. In studying host specificity, we ran growth and pod assays using seventeen pathovars of P. syringae on kidney bean hosts. We tracked bacterial growth numbers over six days and compared pathovar growth patterns. To study immune interactions with host plants, we performed effector-triggered immunity induction and suppression assays with individual effectors in Arabidopsis thaliana to determine whether effector evolutionary age was related to resultant plant immune responses. No correlations were observed. To generate candidate virulence effectors, we sequenced mRNA from seven P. syringae pathovars grown in inducing media and pulled out hits to virulence-related genes.
URI: http://hdl.handle.net/1807/17220
Appears in Collections:Master
Department of Cell and Systems Biology - Master theses

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