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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17236

Title: Design and Evaluation of a Disulphide-crosslinked Hyaluronan Hydrogel for Regeneration of the Intervertebral Disc
Authors: Windisch, Leah Marianne
Advisor: Woodhouse, Kimberly A.
Department: Chemical Engineering and Applied Chemistry
Keywords: hyaluronan hydrogel
elastin-like polypeptide
nucleus pulposus
mechanical response
Issue Date: 26-Feb-2009
Abstract: A cysteine-containing elastin-like polypeptide (ELP2cys) was successfully synthesized and purified, and was shown to behave in a similar fashion to other well-characterized ELPs. Incorporating the ELP2cys as a crosslinking agent into a solution of sulphated hyaluronan (CMHA-S) not only decreased the gelation time of the solution but also increased the crosslinking density of the resultant hydrogel, in turn increasing both the resiliency and stiffness of the construct. Preliminary in vitro work involved culture of human disc cells, followed by their encapsulation within the hydrogel. Unfortunately the results were inconclusive, although it appeared as though the addition of ELP2cys to the matrix did not negatively affect the viability of the cells, as compared to hydrogels with CMHA-S only. This study showed that ELP2cys is a valuable addition to the family of recombinant elastin-like polypeptides, and shows promise as a crosslinking agent in the formation of hyaluronan hydrogels.
URI: http://hdl.handle.net/1807/17236
Appears in Collections:Master
Department of Chemical Engineering and Applied Chemistry - Master theses

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