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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17504

Title: An In Vitro Model System For Cardiac Cell Therapy
Authors: Dengler, Jana
Advisor: Radisic, Milica
Department: Chemical Engineering and Applied Chemistry
Keywords: Cardiac Tissue Engineering
Embryonic Stem Cells
Cell Therapy
In Vitro Model
Issue Date: 7-Aug-2009
Abstract: Embryonic stem cells (ESC) constitute a promising source of cells for cardiac transplantation strategies. However, complexities associated with in vivo studies have made it difficult to develop a thorough understanding of cell integration. We have engineered an in vitro system that recapitulates the native cardiac environment using 300μm thick collagen scaffolds seeded with neonatal cardiomyocytes (CM) and electrical field stimulation. The injection of undifferentiated ESC served as a baseline to assess the validity of studying cell transplantation in this model. Yfp-ESC survived and proliferated over several days in model tissue. ESC were not observed to significantly differentiate into the cardiac lineage, and did not integrate with the cardiac cell population. While the injection of ESC improved cardiac cell number, tissue functional properties were hindered. The methods developed herein can be readily adapted to study ESC derived progenitor and differentiated cells, to elucidate the optimal cell state for ESC-mediated cell therapy.
URI: http://hdl.handle.net/1807/17504
Appears in Collections:Master
Department of Chemical Engineering and Applied Chemistry - Master theses

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