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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17706

Title: Responses of Astrocytes Exposed to Elevated Hydrostatic Pressure and Hypoxia
Authors: Rajabi, Shadi
Advisor: Ethier, C. Ross
Simmons, Craig Alexander
Department: Mechanical and Industrial Engineering
Keywords: Astrocyte
Hypoxia
Elevated Hydrostatic Pressure
Migration
Optic Nerve Head
Issue Date: 22-Sep-2009
Abstract: Several research groups have applied elevated hydrostatic pressure to ONH astrocytes cultured on a rigid substrate as an in vitro model for glaucoma. These studies have shown significant biological effects and this hydrostatic pressure model is now becoming generally accepted in the ophthalmic community. However, since the applied pressures were modest the finding of significant biological effects due to pressure alone is surprising. We hypothesized that the application of hydrostatic pressure as described in these studies also altered gas tensions in the culture media. Our goal was to design equipment and carry out experiments to separate the biologic effects of pressure from those of hypoxia on cultured astrocytes. We designed equipment and carried out experiments to subject cultures of DITNC1 astrocytes to the four combinations of two levels of each parameter. We explored the morphology and migration rates of astrocytes, but observed no significant change in any of these properties.
URI: http://hdl.handle.net/1807/17706
Appears in Collections:Master
Department of Mechanical & Industrial Engineering - Master theses

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