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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17715

Title: An In Vivo Neurophysiological Model of Cortical Ischemia in the Rat
Authors: Srejic, Luka
Advisor: Hutchison, William D.
Aarts, Michelle
Department: Physiology
Keywords: Ischemia
In vivo
Rat
Cortex
Tat-NR2B9c
Microelectrodes
Potentials
Issue Date: 22-Sep-2009
Abstract: Spontaneous and evoked potentials (EPs) were recorded with cross-cortical microelectrode arrays following partial occlusion of the MCA and ACA in urethane-anaesthetised rats. The control group received no occlusion, while the treatment group was injected with anti-stroke peptide Tat-NR2B9c 5min before ischemia. Spontaneous EEG power significantly decreased in the stroke-only group when compared to controls (p<0.001). A greater loss of EEG power was observed on anterior electrodes closer to the occluded area versus posterior contacts in stroke-only rats (p<0.05). The Tat-NR2B9c+stroke group lost significantly less power when compared to stroke-only animals (p<0.05). EP amplitude in the stroke-only group was significantly reduced following ischemia when compared to control and Tat-NR2B9c+stroke animals (p<0.001). Epileptiform discharges were observed in 8/10 untreated stroke rats and 3/5 stroke rats treated with Tat-NR2B9c. The characteristic features of spontaneous and evoked potentials validate this rat focal stroke model for in vivo testing of pharmacological agents.
URI: http://hdl.handle.net/1807/17715
Appears in Collections:Master
Department of Physiology - Master theses

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