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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17876

Title: Role of human neutrophil peptides in the initial interaction between lung epithelial cells and CD4+ lymphocytes.
Authors: Vaschetto, R
Grinstein, J
Del Sorbo, L
Khine, AA
Voglis, S
Tullis, E
Slutsky, AS
Zhang, H
Department: Physiology
St. Michael's Hospital
Keywords: infection
immunity
cytokine
Issue Date: 3-Apr-2007
Publisher: Journal of Leukocyte Biology
Citation: Vaschetto R et al. Role of human neutrophil peptides in the initial interaction between lung epithelial cells and CD4+ lymphocytes. J Leukoc Biol. 2007;81(4):1022-31.
Abstract: Human neutrophil peptides (HNP) exert immune-modulating effects. We hypothesized that HNP link innate and adaptive immunity through activation of costimulatory molecules. Human lung epithelial cells and CD4+ lymphocytes were treated with HNP separately or in coculture. Stimulation with HNP induced an increase in cell surface expression of CD54 (ICAM-1), CD80, and CD86 on lung epithelial cells and the corresponding major ligands, CD11a (LFA-1), CD152 (CTLA-4), and CD28 on CD4+ lymphocytes. There was an increased nuclear expression of the transcription factor p53 in human alveolar A549 cells and an elevated NF-kappaB (p50) and a degradation of I-kappaB protein in CD4+ lymphocytes following HNP stimulation. HNP enhanced the interaction between A549 cells and CD4+ lymphocytes by increasing cell adhesion and release of IFN-gamma, IL-2, and IL-8. This was attenuated by using an alpha1-proteinase inhibitor to neutralize HNP. We conclude that HNP play an important role in linking innate to acquired immunity by activation of costimulatory molecules in lung epithelial cells and CD4+ lymphocytes.
URI: http://dx.doi.org/10.1189/jlb.0706435
http://hdl.handle.net/1807/17876
ISSN: 0741-5400
Appears in Collections:Faculty Publications

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