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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17898

Title: Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen
Authors: Ni, H
Denis, CV
Subbarao, S
Degen, JL
Sato, TN
Hynes, RO
Wagner, DD
Department: Laboratory Medicine and Pathobiology
Physiology
St. Michael's Hospital
Keywords: platelet
thrombus
von Willebrand factor
fibrinogen
Issue Date: 1-Aug-2000
Publisher: American Society for Clinical Investigation
Citation: Ni H et al. Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen. J Clin Invest. 2000;106(3):385-92.
Abstract: We used intravital microscopy to observe the formation of platelet plugs in ferric chloride-injured arterioles of live mice. With this model, we evaluated thrombus growth in mice lacking von Willebrand factor (vWF) and fibrinogen (Fg), the two key ligands known to mediate platelet adhesion and aggregation. In vWF(-/-) mice, despite the presence of arterial shear, delayed platelet adhesion occurred and stable thrombi formed. In many mice, a persisting high-shear channel never occluded. Abundant thrombi formed in Fg(-/-) mice, but they detached from the subendothelium, which ultimately caused downstream occlusion in all cases. Surprisingly, mice deficient in both vWF and Fg successfully formed thrombi with properties characteristic of both mutations, leading to vessel occlusion in the majority of vessels. Platelets of these doubly deficient mice specifically accumulated fibronectin in their alpha-granules, suggesting that fibronectin could be the ligand supporting the platelet aggregation.
URI: http://hdl.handle.net/1807/17898
ISSN: 0021-9738
Appears in Collections:Faculty Publications

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