test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
Faculty of Medicine >
Faculty Publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/17900

Title: Plasma fibronectin promotes thrombus growth and stability in injured arterioles
Authors: Ni, H
Yuen, PS
Papalia, JM
Trevithick, JE
Sakai, T
Fässler, R
Hynes, RO
Wagner, DD
Department: Laboratory Medicine and Pathobiology
Physiology
St. Michael's Hospital
Keywords: fibronectin
thrombus
arterioles
Issue Date: 4-Mar-2003
Publisher: National Academy of Sciences
Citation: Ni H et al. Plasma fibronectin promotes thrombus growth and stability in injured arterioles. Proc Natl Acad Sci U S A. 2003;100(5):2415-9.
Abstract: Mice lacking both of the best-known platelet ligands, von Willebrand factor and fibrinogen, can still form occlusive thrombi in injured arterioles. The platelets of these animals accumulate excessive amounts of fibronectin (FN). These observations led us to examine the contribution of plasma FN (pFN) to thrombus formation. Inactivation of the FN gene in FN conditional knockout mice reduced pFN levels to <2% and platelet FN to approximately 20% of the levels in similarly treated control mice. The mice were then observed in a model of arterial injury to evaluate their capacity to form thrombi. The deficiency of pFN did not affect the initial platelet adhesion, but a delay of several minutes in thrombus formation was observed in the arterioles of pFN-deficient mice as compared with control mice. The thrombi that formed in the absence of pFN were stably anchored to the vessel wall but continuously shed platelets or small platelet clumps, thus slowing their growth significantly; the platelet/platelet cohesion was apparently diminished. Consequently the occlusion of pFN-deficient vessels was delayed, with the majority of vessels remaining patent at the end of the 40-min observation period. We conclude that, in addition to von Willebrand factor and fibrinogen, FN plays a significant role in thrombus initiation, growth, and stability at arterial shear rates and that deficiency in each of the three platelet ligands has its own specific impact on platelet plug formation.
URI: http://hdl.handle.net/1807/17900
ISSN: 0027-8424
Appears in Collections:Faculty Publications

Files in This Item:

File Description SizeFormat
Plasma fibronectin promotes thrombus growth and stability in injured arterioles.pdf185.96 kBAdobe PDF
View/Open

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft