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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18245

Title: The Protein Interactions and Functions of Transient Receptor Potential Melastatin 7 (TRPM7) Ion Channel
Authors: Chan, Chan
Advisor: Aarts, Michelle
Department: Cell and Systems Biology
Keywords: Protein Interactions
TRPM7
Issue Date: 13-Jan-2010
Abstract: Ion channels are proteins that facilitate ion diffusion across cell membrane. Nevertheless, various groups of ion channels can act as surface receptors and play important roles in signal transduction. Transient Receptor Potential Melastatin 7 (TRPM7) ion channel has been implicated in diverse cellular functions including actomyosin cytoskeletal remodeling and anoxic neuronal death. However the mechanisms behind TRPM7’s physiological roles remain undetermined. TRPM7 possesses unusually long intracellular domains and a functional C-terminal alpha kinase domain that may contribute to regulation of channel activity and signal transduction. We therefore identified proteins that interact with TRPM7 C-terminus. Pull-down assays coupled with LC-MS/MS revealed that cytoskeletal proteins (actin and tubulin) and synaptic vesicle proteins (VAMP2 and SNAP25) associate with the TRPM7. In addition, we further found that TRPM7 does not directly bind microtubules or single dimeric tubulin subunits. Thus one or more microtubule binding proteins is involved in the association between TRPM7 and microtubules.
URI: http://hdl.handle.net/1807/18245
Appears in Collections:Master
Department of Cell and Systems Biology - Master theses

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