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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18266

Title: Interactions of Surfactant Protein D with the Glycoproteins Ovalbumin and Alpha-2-Macroglobulin
Authors: Craig-Barnes, Hayley A.
Advisor: Palaniyar, Nades
Department: Laboratory Medicine and Pathobiology
Keywords: Surfactant Protein D
Innate Immunity
Issue Date: 13-Jan-2010
Abstract: Surfactant protein D (SP-D) is an important innate immune collectin involved in uptake and clearance of microbes and allergens in the lungs. SP-D has been shown to ameliorate allergic asthma reactions in mice; however, the mechanisms for this are not fully understood. We investigated the role of SP-D in the uptake and clearance of the model allergen ovalbumin (OVA) by macrophages. We discovered that SP-D does not bind OVA but binds fractions with contaminating proteins; ovomucin and ovomacroglobulin. We extended these findings to show that SP-D binds human alpha-2-macroglobulin (A2M) in its cleaved or intact state, in a concentration-, calcium-, and carbohydrate-dependent manner. A2M increases the innate immune potential of SP-D by increasing its ability to agglutinate the bacteria Escherichia coli and Bacillus subtilis. We found that SP-D does not increase the uptake of OVA by murine macrophage cell lines, or by alveolar macrophages in vivo in BALB/cJ mice.
URI: http://hdl.handle.net/1807/18266
Appears in Collections:Master
Department of Laboratory Medicine and Pathobiology - Master theses

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