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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18287

Title: Single Molecule Imaging of Membrane Proteins: A study of the CorA Transporter by Scanning Probe Microscopy
Authors: El Masri, Ghaleb
Advisor: Yip, Christopher M.
Department: Biochemistry
Keywords: membrane protein
AFM
Issue Date: 15-Jan-2010
Abstract: Elucidating the structure-function relationships of membrane proteins is critical for the design of therapeutic agents to treat disease and for understanding numerous cellular processes such as signal transduction and molecular or ion transport. Recent advances in the application of correlated single molecule imaging techniques have provided new insights into protein-protein and protein-membrane interactions. To demonstrate the potential of these approaches, we have used in situ atomic force microscopy and single molecule fluorescence microscopy to characterize the interactions between membrane receptors and their soluble ligands, examine the monomer-dimer equilibrium in a family of adhesion receptors, and elucidate protein-mediated membrane restructuring of a supported lipid bilayer. Building on these studies, we examined the CorA ion transporter protein. We demonstrated single molecule resolution of reconstituted CorA molecules in supported lipid bilayers using a correlated AFM-TIRF microscopy platform. This approach provided new insights into a purported mechanism of CorA activation that involved ion binding.
URI: http://hdl.handle.net/1807/18287
Appears in Collections:Master
Department of Biochemistry - Master theses

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