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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18762

Title: The Regulation of Juvenile Hormone in Dictyoptera: A Functional and Evolutionary Study of USP/RXR and Allatostatin
Authors: Hult, Ekaterina F.
Advisor: Tobe, Stephen
Department: Cell and Systems Biology
Keywords: juvenile hormone
Diploptera punctata
retinoid X receptor
Issue Date: 12-Feb-2010
Abstract: The objective of this study was to clarify the regulation of production and signal transduction of juvenile hormone (JH) in insects by experimentally examining the function and evolution of a putative receptor (USP/RXR) and a neuropeptide inhibitor (FGLamide allatostatin). To examine the role of USP/RXR, the cDNA sequence of the receptor was obtained from the cockroach Diploptera punctata. Transcript levels during developmentally critical periods for JH sensitivity may suggest USP/RXR is JH responsive. Comparative sequence analysis of evolutionary rates in the Mecopterida support current hypotheses which suggest some gain in function along this lineage, although this acquisition may have occurred more gradually than previously assumed. To examine allatostatin evolution within insects, ancestral peptides inferred using maximum likelihood ancestral reconstruction methods were assayed for in vitro inhibition of JH production in two cockroach species. Shifts in peptide potency in some ancestral peptides reconstructed may be related to peptide copy number evolution.
URI: http://hdl.handle.net/1807/18762
Appears in Collections:Master
Department of Cell and Systems Biology - Master theses

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