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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18787

Title: Chloride Channel 2 and Protein Kinase C Epsilon Protein Module in Ischemic Preconditioning of Rabbit Cardiomyocytes
Authors: Kuzmin, Elena
Advisor: Wilson, Gregory J.
Department: Laboratory Medicine and Pathobiology
Keywords: protein kinase c epsilon
chloride channel 2
Issue Date: 12-Feb-2010
Abstract: Cardiac ischemic preconditioning (IPC) is defined as brief periods of ischemia and reperfusion that protect the heart against longer ischemia and reperfusion. IPC triggers Cl- efflux and protein kinase C epsilon (PKCe) translocation to the particulate fraction. Chloride channel 2 (ClC-2) is volume regulated and is a potential end effector of IPC. The goal of my study was to investigate the involvement of PKCĪµ and ClC-2 protein module in IPC of isolated adult rabbit ventricular myocytes. Co-immunoprecipitation (co-IP) assays on HEK 293 cells, transfected with ClC-2-Flag, confirmed that ClC-2 interacts with PKCe. Subcellular fractionation showed that PKCe/ClC-2 protein module is localized to the sarcolemma of cardiomyocytes. Lastly, ischemia/reperfusion injury was simulated in cardiomyocytes with 45min simulated ischemia (SI)/60min simulated reperfusion (SR) and IPC was induced by pre-treatment with 10min SI/20min SR. Co-IP after each time interval showed that IPC transiently enhanced PKCe/ClC-2 interaction. PKC inhibitor, GF109203X, abrogated the enhanced interaction.
URI: http://hdl.handle.net/1807/18787
Appears in Collections:Master
Department of Laboratory Medicine and Pathobiology - Master theses

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