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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18828

Title: The Effect of Glucagon-like Peptide-2 on Insulin-like Growth Factor-1 in Murine Intestinal Subepithelial Myofibroblasts
Authors: Leen, Jason
Advisor: Brubaker, Patricia
Department: Physiology
Keywords: Cell cultures
Glucagon-like peptide-2
Subepithelial myofibroblasts
Insulin-like growth factor-1
PI3K/AKT signaling
Intestinal growth
Real time RT-PCR
Western blots
Immunocytochemistry
RIA
Issue Date: 15-Feb-2010
Abstract: Insulin-like growth factor-1 (IGF-1), a known secretory product of intestinal subepithelial myofibroblasts (ISEMF), is essential for the intestinotrophic effects of glucagon-like peptide-2(GLP-2). I hypothesized that GLP-2 increases the production of IGF-1 by primary murine ISEMF in culture. Immunocytochemistry showed that the ISEMF stained appropriately for α smooth muscle actin and vimentin but not for desmin. The ISEMF also expressed GLP-2 receptor and IGF-1 mRNA transcripts. ISEMF treated with GLP-2 revealed a maximal increase in IGF-1 mRNA transcript levels at 10-8 M GLP-2 and 2hr. Interestingly, immunoblotting revealed an increase in P-AKT/T-AKT with GLP-2, but no changes in cAMP, P-ERK/T-ERK or calcium were detected. PI3K inhibition and kinase-dead AKT over-expression abrogated GLP-2-induction of IGF-1 mRNA, and ISEMF from GLP-2R null mice demonstrated reductions in IGF-1 mRNA and cellular IGF-1, but not in media IGF-1, vs. wild-type ISEMF. These findings suggest a possible mechanism by which GLP-2 increases intestinal growth in-vivo.
URI: http://hdl.handle.net/1807/18828
Appears in Collections:Master
Department of Physiology - Master theses

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