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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18867

Title: Characterization of the Novel Cysteine-rich Extracellular Calmodulin-binding Protein cyrA from Dictyostelium discoideum
Authors: Suarez, Andres
Advisor: O'Day, Danton H.
Department: Cell and Systems Biology
Keywords: calmodulin-binding protein
Dictyostelium
extracellular
matricellular
epidermal growth factor-like
cysteine rich
proteolytic processing
secreted
calmodulin
cyrA
Issue Date: 15-Feb-2010
Abstract: A novel calmodulin (CaM)-binding cysteine-rich protein from Dictyostelium, cyrA, with epidermal growth factor-like (EGFL) repeats was discovered and characterized. Calcium-dependent and –independent CaM-binding was verified. Western blots show that full length cyrA is detected constitutively throughout development. Analyses of the extracellular medium reveal that cyrA is cleaved and that the fragments containing the N-terminus are secreted early in development, while those containing the C-terminus are secreted later. In support of this, GFP and immunohistochemistry studies reveal that cyrA localizes to the endoplasmic reticulum and secretory vesicles of vegetative cells, and to the extracellular matrix (slime sheath) of migrating slugs. The addition of EGFL1 peptides enhanced cell motility and cAMP-mediated chemotaxis. Finally, cyrA cleavage is regulated by extracellular Dictyostelium CaM and by the extracellular EGFL repeats. In total the data suggest that cyrA is a true matricellular protein that mediates cell motility during multicellular development.
URI: http://hdl.handle.net/1807/18867
Appears in Collections:Master
Department of Cell and Systems Biology - Master theses

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