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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/18955

Title: Programmed Cell Death 4 is a Direct Target of miR-21 and Regulates Invasion in Oral Squamous Cell Carcinoma
Authors: Tomenson, Miranda
Advisor: Kamel-Reid, Suzanne
Department: Medical Biophysics
Keywords: Oral Cancer
PDCD4
miR-21
Metastasis
Invasion
Biomarkers
Issue Date: 16-Feb-2010
Abstract: Programmed Cell Death 4 (PDCD4) is a known tumour suppressor, lost in carcinomas of the breast, prostate, colon, lung and ovary. This study found significantly reduced levels of PDCD4 mRNA and protein in both primary patient oral squamous cell carcinomas (OSCCs) and OSCC cell lines. Moreover, lower PDCD4 mRNA levels were significantly correlated with nodal metastasis (P=0.019). To determine the functional significance of PDCD4 down-regulation in OSCC we asked whether PDCD4 played a role in invasion. In fact, over-expression of PDCD4 decreased invasion of OSCC lines. We then sought to determine a mechanism for PDCD4 down-regulation in OSCC. Previous studies in breast and colon carcinomas suggested that reduced PDCD4 expression was due to over-expression of miR-21. Interestingly, miR-21 was inversely correlated to PDCD4 mRNA (P=0.002) and PDCD4 protein (P<0.001) levels in OSCC patient samples. Moreover, we found that miR-21 directly regulated PDCD4 protein expression in OSCC cell lines. This is the first report in OSCC that demonstrates that PDCD4 is down-regulated by miR-21 and may play a role in OSCC invasion.
URI: http://hdl.handle.net/1807/18955
Appears in Collections:Master
Department of Medical Biophysics - Master theses

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