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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/19008

Title: Mitochondria-penetrating Peptides: Characterization and Cargo Delivery
Authors: Yousif, Lema F.
Advisor: Kelley, Shana O.
Department: Biochemistry
Keywords: mitochondria
peptide transporter
drug delivery
copper delivery
Issue Date: 17-Feb-2010
Abstract: A class of mitochondria-penetrating peptides (MPPs) was studied in an effort to optimize their applications in the delivery of bioactive cargo to this therapeutically important organelle. The sequence requirements for mitochondrial entry were monitored, and it was discovered that while an alternating cationic/hydrophobic residue motif is not required, the inclusion of a stretch of adjacent cationic amino acids can impede access to the organelle. In addition, a variety of C-terminal cargos were tested to determine if there are limitations to the lipophilicity, charge, or polarity of compounds that can be transported to mitochondria by MPPs. Furthermore, these systematic studies aided the design and synthesis of a copper-binding MPP for the delivery of copper ions to mitochondria for the potential rescue of disorders associated with copper-deficiency. The results reported demonstrate that MPPs are versatile transporters that may have a wide range of biological applications.
URI: http://hdl.handle.net/1807/19008
Appears in Collections:Master
Department of Biochemistry - Master theses

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