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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/19505


Title: Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
Authors: Nacife, Valéria Pereira
Meirelles, Maria de Nazareth Leal de
Filho, Fernando Costa e Silva
Keywords: monocytes - cell surface - surface charge - surface tension - Didelphis marsupialis
Issue Date: 31-Dec-1998
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 93 Num 6
Abstract: The electrostatic surface charge and surface tension of mononuclear cells/monocytes obtained from young and adult marsupials (Didelphis marsupialis) were investigated by using cationized ferritin and colloidal iron hydroxyde, whole cell electrophoresis, and measurements of contact angles. Anionic sites were found distributed throughout the entire investigated cell surfaces. The results revealed that the anionic character of the cells is given by electrostatic charges corresponding to -18.8 mV (cells from young animals) and -29.3 mV (cells from adult animals). The surface electrostatic charge decreased from 10 to 65.2% after treatment of the cells with each one of trypsin, neuraminidase and phospholipase C. The hydrophobic nature of the mononuclear cell surfaces studied by using the contact angle method revealed that both young and adult cells possess cell surfaces of high hidrofilicity since the angles formed with drops of saline water were 42.5and 40.8, respectively. Treatment of the cells with trypsin or neuraminidase rendered their surfaces more hydrophobic, suggesting that sialic acid-containing glycoproteins are responsible for most of the hydrophilicity observed in the mononuclear cell surfaces from D. marsupialis.
URI: http://hdl.handle.net/1807/19505
Other Identifiers: http://www.bioline.org.br/abstract?id=oc98215
Rights: Copyright 1998 Fundacao Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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