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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/2001

Title: Detection by polymerase chain reaction of enterovirus-like sequence in patience with epidemic neuropathy in Cuba
Authors: Alvarez, Maritza
Muzio, Verena
Marrero, Miguel
Barro, Mario
Rosario, Delfina
Jomarron, Liliana
Martin, Alejandro
Sariol, Carlos
Candelario, Mayda
Maestre, Jorge
de la Fuente, Jose
Herrera, Luis
Guzman, Maria
Mas, Pedro
Kouri, Gustavo
Keywords: Biotechnology
PCR, Epidemic Neuropathy, enterovirus ba95008
Issue Date: 1995
Publisher: Elfos Scientiae
Citation: Biotecnologia Aplicada 12(1)
Abstract: Enterovirus-like sequences were found in patients with epidemic neuropathy (EN) in Cuba. We examined cerebrospinal fluids (CSFs) and nerve biopsy or necropsy specimens for the presence of Enterovirus RNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Specific amplification was detected in 40 out of 111 CSFs from patients with EN and in 1 out of 13 control CSFs. In the two optical nerves examined from patients with EN, positive signals were obtained by RT-PCR. A cytopathic effect (CPE) was shown in the CSF from patients positive by RT-PCR and in 43 negative ones. Two of these agents caused enterovirus-like (Cox A9) CPE and the rest showed a CPE atypical of enteroviruses. The possible role of enteroviruses in the etiology and pathogenesis of EN is not known. RESUMEN Se detectaron secuencias homologas con enterovirus en pacientes con neuropatia epidemica (EN) en Cuba. Se examinaron muestras de liquido cefalorraquideo (CSFs), biopsias de nervios y especimenes de necropcias para la presencia de ARN de enterovirus empleando la reaccion de reverso-transcripcion-reaccion en cadena de la polimerasa (RT-PCR). Se detecto amplificacion especifica en 40 de las 111 muestras de CSFs de pacientes con EN y en una de 13 controles. En dos nervios opticos de pacientes con EN examinados se detecto ARN homologo a enterovirus mediante RT-PCR. Se demostro la presencia de efecto citopatico (CPE) en los CSFs positivos por RT-PCR y en 43 muestras que resultaron negativas por RT-PCR. Dos de estos agentes causaron un CPE tipico de enterovirus (coxA9) y el resto mostro un CPE diferente al caracteristico para enterovirus. El posible papel de enterovirus en la etiopatogenia de la EN no se conoce.
URI: http://bioline.utsc.utoronto.ca/archive/00000999/01/ba95008.pdf
Appears in Collections:Bioline International Legacy Collection

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