|Title: ||Comparative Evaluation of a Simple and Sensitive Assay for Detection of Orthomyxo and Paramyxoviruses|
|Authors: ||Couceiro, J. N. S. S.|
Couceiro, E. S. S.
Pinto, A. M. V.
Machado, R. D.
Cabral, M. C.
|Keywords: ||hemagglutination assay - lectin-neuraminidase assay - influenzaviruses
|Issue Date: ||31-Dec-1994|
|Publisher: ||Fundação Oswaldo Cruz, Fiocruz|
|Citation: ||Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 89 Num 2|
|Abstract: ||Studies were done to evaluate comparatively the traditional HA assay
and a more recently introduced lectin-neuraminidase (LN) methodology in
search of a simple and sensitive assay for virus detection during
laboratorial diagnosis. The results proved the value of LN assay as a
sensitive methodology for detection of virus particles, presenting
results at least equal to those obtained by HA (hemagglutination)
assay, with significant values of accumulated frequencies for LN/HA
factors (ratios between LN and HA titers) higher than two. The
accumulated values of frequencies for LN/HA factors as high as four
were very significant, 72.7% for influenzavirus and 60.7% for Newcastle
disease virus (NDV), moreover accumulated frequencies for LN/HA factors
even as high as 32 were due to influenzavirus (45.4%) and NDV (7.2%)
samples. After the storage period, most of those concentraded samples
that even did not present HA titers could be detected through LN assay,
demonstrating a lower threshold for virus detection.|
|Other Identifiers: ||http://www.bioline.org.br/abstract?id=oc94038|
|Rights: ||Copyright 1994 Memorias do Instituto Oswaldo Cruz.|
|Appears in Collections:||Bioline International Legacy Collection|