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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20023


Title: Comparative Evaluation of a Simple and Sensitive Assay for Detection of Orthomyxo and Paramyxoviruses
Authors: Couceiro, J. N. S. S.
Couceiro, E. S. S.
Pinto, A. M. V.
Machado, R. D.
Cabral, M. C.
Keywords: hemagglutination assay - lectin-neuraminidase assay - influenzaviruses - parainfluenzaviruses
Issue Date: 31-Dec-1994
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 89 Num 2
Abstract: Studies were done to evaluate comparatively the traditional HA assay and a more recently introduced lectin-neuraminidase (LN) methodology in search of a simple and sensitive assay for virus detection during laboratorial diagnosis. The results proved the value of LN assay as a sensitive methodology for detection of virus particles, presenting results at least equal to those obtained by HA (hemagglutination) assay, with significant values of accumulated frequencies for LN/HA factors (ratios between LN and HA titers) higher than two. The accumulated values of frequencies for LN/HA factors as high as four were very significant, 72.7% for influenzavirus and 60.7% for Newcastle disease virus (NDV), moreover accumulated frequencies for LN/HA factors even as high as 32 were due to influenzavirus (45.4%) and NDV (7.2%) samples. After the storage period, most of those concentraded samples that even did not present HA titers could be detected through LN assay, demonstrating a lower threshold for virus detection.
URI: http://hdl.handle.net/1807/20023
Other Identifiers: http://www.bioline.org.br/abstract?id=oc94038
Rights: Copyright 1994 Memorias do Instituto Oswaldo Cruz.
Appears in Collections:Bioline International Legacy Collection

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