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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20231

Title: Serodiagnosis of Trypanosoma cruzi infection using the new particle gel immunoassay - ID-PaGIA Chagas
Authors: Rabello, Ana Lúcia Teles
Luquetti, Alejandro O.
Moreira, Eliana F.
Gadelha, Maria de Fátima
Alisson dos Santos, José
Melo, Laércio de
Schwind, Peter
Keywords: Chagas' disease - immunodiagnosis - serological tests-particle gel immunoassay
Issue Date: 31-Dec-1999
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 94 Num 1
Abstract: The ID-Chagas test is a particle gel immunoassay (PaGIA). Red coloured particles are sensitised with three different synthetic peptides representing antigen sequences of Trypanosoma cruzi: Ag2, TcD and TcE. When these particles are mixed with serum containing specific antibodies, they agglutinate. The reaction mixture is centrifuged through a gel filtration matrix allowing free agglutinated particles to remain trapped on the top or distributed within the gel. The result can be read visually. In order to investigate the ability of the ID-PaGIA to discriminate negative and positive sera, 111 negative and 119 positive, collected in four different Brazilian institutions, were tested by each of the participants. All sera were previously classified as positive or negative according to results obtained with three conventional tests (indirect immunofluorescence, indirect hemaglutination, and enzime linked immunosorbent assay). Sensitivity rates of ID-PaGIA varied from 95.7% to 97.4% with mean sensitivity of 96.8% and specificity rates varied from 93.8 to 98.8% with mean specificity of 94.6%. The overall Kappa test was 0.94. The assay presents as advantages the simplicity of operation and the reaction time of 20 min. In this study, ID-PaGIA showed to be highly sensitive and specific.
URI: http://hdl.handle.net/1807/20231
Other Identifiers: http://www.bioline.org.br/abstract?id=oc99016
Rights: Copyright 1999 Fundacao Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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