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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20353

Title: Ultrastructural, Antigenic and Physicochemical Characterization of the Mojuí dos Campos (Bunyavirus) Isolated from Bat in the Brazilian Amazon Region
Authors: Wanzeller, Ana L. M.
Diniz, José A. P.
Gomes, Maria L. C.
Cruz, Ana C. R.
Soares, Manoel C. P.
de Souza, Wanderley
Travassos da Rosa, Amélia P. A.
Vasconcelos, Pedro F. C.
Keywords: arbovirus - Mojuí dos Campos virus - characterization - ultrastructure - protein kinetics - Amazon region - Brazil
Issue Date: 31-Dec-2002
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 97 Num 2
Abstract: The Mojuí dos Campos virus (MDCV) was isolated from the blood of an unidentified bat (Chiroptera) captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i), and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup.
URI: http://hdl.handle.net/1807/20353
Other Identifiers: http://www.bioline.org.br/abstract?id=oc02059
Rights: Copyright 2002 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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