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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20850

Title: Sequencing and expression analysis of a Schistosoma mansoni gene homologue to a Drosophila gene involved in germ plasm assembly
Authors: Rabelo, Élida Mara Leite
Hobaika, Adriano B. S.
Coelho, Paulo Marcos Z.
Pena, Sérgio Danilo Junho
Keywords: Schistosoma mansoni - mago nashi - germ plasm - gene characterization - sequencing
Issue Date: 31-Dec-1998
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 93 Num s1
Abstract: Genes coding for proteins involved in gene regulation and/or development are of great interest in the study of the biology of Schistosoma mansoni. This trematode is the etiologic agent of schistosomiasis and presents a complex life cycle with drastic morphologic changes between stages. Recently, some strains have become resistant to the drugs currently in use to eradicate the disease. The strategy of gene discovery program in S. mansoni by using the EST (expressed sequence tag) approach has been very efficient in the discovery of new S. mansoni genes, which were unlikely to be identified using classical procedures based on phenotype. A class of genes that interested us particularly were those that in other organisms were known to be involved in the regulation of embryogenesis. Among these, we selected one that presented a high homology to a Drosophila gene named mago nashi. In diptera this gene is involved in the process of germ plasm assembly and its mutation results in sterility of F1 progeny and also in the formation of the perpendicular axes. We reasoned that this gene might conceivably play a role in the morphogenetic changes seen in the life cycle of the parasite. We thus decided to characterize S. mansoni mago nashi further by obtaining its full length cDNA and genomic sequences, as well as studying its expression pattern at the different life stages of the worm.
URI: http://hdl.handle.net/1807/20850
Other Identifiers: http://www.bioline.org.br/abstract?id=oc98169
Rights: Copyright 1998 Fundacao Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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