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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20852


Title: Cytotoxic and Antiviral Activities of Colombian Medicinal Plant Extracts of the Euphorbia genus
Authors: Betancur-Galvis, L. A.
Morales, G. E.
Forero, J. E.
Roldan, J.
Keywords: Euphorbia   ceae - ethnobotany - medicinal plants - antiviral - herpes simplex virus - Colombia
Issue Date: 31-Dec-2002
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 97 Num 4
Abstract: Forty-seven plant extracts of 10 species of the genus Euphorbia   ( Euphorbia ceae) used by Colombian traditional healers for the treatment of ulcers, cancers, tumors, warts, and other diseases, were tested in vitro for their potential antitumour (antiproliferative and cytotoxic) and antiherpetic activity. To evaluate the capacity of the extracts to inhibit the lytic activity of herpes simplex virus type 2 (HSV-2) and the reduction of viability of infected or uninfected cell cultures, the end-point titration technique (EPTT) and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay were used, respectively. The therapeutic index of the positive extracts for the antiviral activity was determined by calculating the ratio CC 50 (50% cytotoxic concentration) over IC 50 (50% inhibitory concentration of the viral effect). Five of the 47 extracts (11%) representing 3 out of 10 Euphorbia species (30%) exhibited antiherpetic action; the highest activity was found in the leaf/stem water-methanol extracts from E. cotinifolia   and E. tirucalli   . The therapeutic indexes of these two plant species were > 7.1; these extracts exhibited no cytotoxicity. Six extracts (13%) representing 4 plant species (40%) showed cytotoxic activity. The highest cytotoxicity was found in the dichloromethane extract obtained from E. cotinifolia leaves and the CC 50 values for the most susceptible cell lines, HEp-2 and CHO, were 35.1 and 18.1 mg/ml, respectively.
URI: http://hdl.handle.net/1807/20852
Other Identifiers: http://www.bioline.org.br/abstract?id=oc02103
Rights: Copyright 2002 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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