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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20966


Title: Leishmanial antigens in the diagnosis of active lesions and ancient scars of American tegumentary leishmaniasis patients
Authors: Schubach, Armando
Cuzzi-Maya, Tullia
Oliveira, Albanita V.
Sartori, Alexandrina
P. de Oliveira-Neto, Manoel
Mattos, Marise S.
Araújo, Marcelo Lodi
Souza, Wilson Jacinto S.
Haddad, Fátima
Perez, Maurício de A.
Pacheco, Raquel S.
Momen, Hooman
Coutinho, Sérgio G.
Marzochi, Mauro Célio de Almeida
Marzochi, Keyla Belizia Feldman
Gonçalves da Costa, Sylvio Celso
Keywords: American tegumentary leishmaniasis, Leishmania (Viannia) braziliensis, immunodiagnosis, scar, cured patients
Issue Date: 31-Dec-2001
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 96 Num 7
Abstract: Cutaneous biopsies (n = 94) obtained from 88 patients with American tegumentary leishmaniasis were studied by conventional and immunohistochemical techniques. Specimens were distributed as active lesions of cutaneous leishmaniasis (n = 53) (Group I), cicatricial lesions of cutaneous leishmaniasis (n = 35) (Group II) and suggestive scars of healed mucosal leishmaniasis patients (n = 6) (Group III). In addition, active cutaneous lesions of other etiology (n = 24) (Group C1) and cutaneous scars not related to leishmaniasis (n = 10) (Group C2) were also included in the protocol. Amastigotes in Group I biopsies were detected by routine histopathological exam (30.2%), imprint (28.2%), culture (43.4%), immunofluorescence (41.4%) and immunoperoxidase (58.5%) techniques; and by the five methods together (79.3%). In Group II, 5.7% of cultures were positive. Leishmanial antigen was also seen in the cytoplasm of macrophages and giant cells (cellular pattern), vessel walls (vascular pattern) and dermal nerves (neural pattern). Positive reaction was detected in 49 (92.5%), 20 (57%) and 4 (67%) biopsies of Groups I, II and III, respectively. Antigen persistency in cicatricial tissue may be related to immunoprotection or, on the contrary, to the development of late lesions. We suggest that the cellular, vascular and neural patterns could be applied in the immunodiagnosis of active and cicatricial lesions in which leishmaniasis is suspected.
URI: http://hdl.handle.net/1807/20966
Other Identifiers: http://www.bioline.org.br/abstract?id=oc01192
Rights: Copyright 2001 Instituto Oswaldo Cruz - Fiocruz.
Appears in Collections:Bioline International Legacy Collection

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