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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20980


Title: The Zymovars of Vibrio cholerae   : Multilocus Enzyme Electrophoresis of Vibrio cholerae
Authors: Freitas, Fernanda S.
Momen, Hooman
Salles, Carlos Andre
Keywords: Vibrio cholerae   - enzyme electrophoresis - genetic diversity
Issue Date: 31-Dec-2002
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 97 Num 4
Abstract: Zymovars analysis also known as multilocus enzyme electrophoresis is applied here to investigate the genetic variation of Vibrio cholerae   strains and characterise strains or group of strains of medical and epidemiological interest. Fourteen loci were analyzed in 171 strains of non-O1 non-O139, 32 classical and 61 El Tor from America, Africa, Europe and Asia. The mean genetic diversity was 0.339. It is shown that the same O antigen (both O1 and non-O1) may be present in several geneticaly diverse (different zymovars) strains. Conversely the same zymovar may contain more than one serogroup. It is confirmed that the South American epidemic strain differs from the 7th pandemic El Tor strain in locus LAP (leucyl leucyl aminopeptidase). Here it is shown that this rare allele is present in 1 V. mimicus and 4 non-O1 V. cholerae. Non toxigenic O1 strains from South India epidemic share zymovar 14A with the epidemic El Tor from the 7th pandemic, while another group have diverse zymovars. The sucrose negative epidemic strains isolated in French Guiana and Brazil have the same zymovar of the current American epidemic V. cholerae
URI: http://hdl.handle.net/1807/20980
Other Identifiers: http://www.bioline.org.br/abstract?id=oc02097
Rights: Copyright 2002 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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