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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/20997

Title: Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa   to a hospital disinfectant and molecular typing
Authors: Romão, Célia Maria Carvalho Pereira Araujo
Faria, Yaisa Naziozeno
Pereira, Luciana Roberto
Asensi, Marise Dutra
Keywords: Pseudomonas aeruginosa - disinfectants - pulsed field gel electrophoresis
Issue Date: 31-Dec-2005
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 100 Num 5
Abstract: The aim of this study was to evaluate the susceptibility of 35 resistant Pseudomonas aeruginosa   clinical isolates to a quaternary ammonium hospital disinfectant. The methodology was the AOAC Use-Dilution Test, with disinfectant at its use-concentration. In addition, the chromosomal DNA profile of the isolates were determined by macro-restriction pulsed field gel electrophoresis (PFGE) method aiming to verify the relatedness among them and the behavior of isolates from the same group regarding the susceptibility to the disinfectant. Seventy one percent of the isolates were multiresistant to antibiotics and 43% showed a reduced susceptibility to the disinfectant. The PFGE methodology detected 18 major clonal groups. We found isolates with reduced susceptibility to the disinfectant and we think that these are worrying data that should be further investigated including different organisms and chemical agents in order to demonstrate that microorganisms can be destroyed by biocide as necessary. We also found strains of the same clonal groups showing different susceptibility to the disinfectant. This is an interesting observation considering that only few works are available about this subject. PFGE profile seems not to be a reliable marker for resistance to disinfectants.
URI: http://hdl.handle.net/1807/20997
Other Identifiers: http://www.bioline.org.br/abstract?id=oc050127
Rights: Copyright 2005 - Instituto Oswaldo Cruz - Fiocruz.
Appears in Collections:Bioline International Legacy Collection

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