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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/21729


Title: The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
Authors: Leao, Ana Maria dos A. Carneiro
de Carvalho Junior, Luiz Bezerra
Malagueño, Elizabeth
Keywords: magnetic ELISA - ferromagnetic dacron - human serum albumin
Issue Date: 31-Dec-1994
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 89 Num 2
Abstract: Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalently immobilized onto ferromagnetic dacron and an enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o- phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 um (diameter) and 10 ug of immobilized antigen were used. Positive reactions were detected until dilutions of 1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
URI: http://hdl.handle.net/1807/21729
Other Identifiers: http://www.bioline.org.br/abstract?id=oc94039
Rights: Copyright 1994 Memorias do Instituto Oswaldo Cruz.
Appears in Collections:Bioline International Legacy Collection

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