test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
University of Toronto at Scarborough >
Bioline International Legacy Collection >
Bioline International Legacy Collection >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/21731


Title: NOTA TÉCNICA - Determination of saxitoxin and its derivatives in mollusk bivalves: Evaluation and optimization of the high performance liquid chromatographic use with fluorescence detection and prior oxidation (Determinación de saxitoxina y sus derivados en moluscos bivalvos: Evaluación y optimización del uso de cromatografía líquida de alta resolución con previa oxidación)
Authors: Rojas de Astudillo, Luisa
Chang-Yen, Iván
La Barbera-Sánchez, Amelia
Keywords: saxitoxin, PSP, neurotoxin, dinoflagellate, toxic
Saxitoxina, PSP, neurotoxina, dinoflagelados, tóxicos.
Issue Date: 31-Dec-2002
Publisher: Instituto Nacional de Investigaciones Agrícolas Venezuela
Citation: Zootecnia Tropical (ISSN: 0798-7269) Vol 20 Num 4
Abstract: Saxitoxin and its derivatives are potent neurotoxins associated with paralytic shellfish poisoning (PSP) in humans which are primarily produced by dinoflagellates. To evaluate and optimize the use of high performance liquid chromatography (HPLC) with previous oxidation in order to determine saxitoxin and its derivatives, samples of the green mussel (Perna viridis   ) and oysters (Crassostrea   sp) were collected at six locations in Venezuela and at four in Trinidad, the latter along the west coast line, in June 2000. The samples were extracted using the AOAC bioassay mouse procedure. Each extract was oxidized using periodic acid and the oxidation product was injected to the chromatographic column. The method used to HPLC in this work was a modification of Lawrence et al. (1996). The separation of important pairs of toxins (STX/NEO, GTX1,4/GTX2,3) were achieved, but the resolution and height of the peaks of oxidation products of these toxins varied depending on column efficiencies. Some samples had presence of PSP below permissible levels, which was confirmed by using bioassay mouse method. The results showed that the reaction precolumn method gives prompt information about profile of the toxins, which is independent of matrix of receptor organism, and it is a simple method for monitoring PSP in shellfish in the Caribbean.
URI: http://hdl.handle.net/1807/21731
Other Identifiers: http://www.bioline.org.br/abstract?id=zt02033
Rights: Copyright 2002 - Zootecnia Tropical. Free full-text also available online at http://www.ceniap.gov.ve/bdigital/ztzoo/ztindice.htm
Appears in Collections:Bioline International Legacy Collection

Files in This Item:

File Description SizeFormat
zt02033.html89.98 kBHTMLView/Open

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft