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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/21796

Title: Expression of recombinant proteins in the milk of transgenic mice and rabbits. Implications for the use of transgenic rabbits as bioreactors
Issue Date: 31-Dec-1996
Publisher: Elfos Scientiae
Citation: Biotecnologia Aplicada (ISSN: 0684-4551) Vol 13 Num 1
Abstract: Biotecnologia Aplicada 1996 Volume 3 No. 1 Expression of recombinant proteins in the milk of transgenic mice and rabbits. Implications for the use of transgenic rabbits as bioreactors Fidel Ovidio Castro, Jose de la Fuente, Alina Rodriguez, Jose Limonta, Eileen Riego, Alina Aguirre, Boris Ramos, Anselmo Aguilar, Dagmara Pichardo, Pedro Puentes and Diana Garcia Code Number:BA96008 Size of Files: Text: 4.8K No associated graphics files Recent advances in mammalian gene transfer, had made it possible to produce transgenic animals with new functions. Among these is specially remarkable the targeting of transgene expression to the mammary gland. Transgenic proteins are thus secreted to the milk during lactation. The optimal end point of the above mentioned process will be the expression of the foreign gene at levels similar to those of the endogenous milk genes. At present, transgenic proteins have been expressed in the milk of transgenic mice, rats, rabbits, pigs, goats, sheep and cattle. While transgenesis in rodents is easy to acchieve, and relatively cheap, these animals can be used mainly for testing gene constructs and research purposes. Transgenesis in farm animals is handicaped by several factors ranging from difficulties in embryo availability, long reproductive intervals and lower efficiciency of transgenesis. Furthermore the cost of transgenesis in farm animals is rather high. Rabbits are indistinctively used as laboratory and farm animals. However, from the point of view of transgenesis, rabbits offer several advantages over both laboratory and farm animals; small size and relative low cost of manteinance, short reproductive cycles; high embryos yield, average milk yield of around 20 litters per doe per year. As a major drawback, not every transgene can be expressed in rabbit milk, but only those that are needed in small amounts. Copyright 1996 Elfos Scientiae
URI: http://hdl.handle.net/1807/21796
Other Identifiers: http://www.bioline.org.br/abstract?id=ba96008
Rights: Copyright 1996 Elfos Scientiae
Appears in Collections:Bioline International Legacy Collection

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