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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/21807


Title: Kinetics of T cell-activation molecules in response to mycobacterium tuberculosis antigens
Authors: Antas, Paulo R. Z.
Oliveira, Eliane B.
Milagres, Alexandre S.
Franken, Kees C.
Ottenhoff, Tom H. M.
Klatser, Paul R.
Sarno, Euzenir N.
Sampaio, Elizabeth P.
Keywords: activation markers - CD69 - CD25 - flow cytometry
Issue Date: 31-Dec-2002
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 97 Num 8
Abstract: The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease.
URI: http://hdl.handle.net/1807/21807
Other Identifiers: http://www.bioline.org.br/abstract?id=oc02240
Rights: Copyright 2002 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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