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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/22040


Title: Alternative Respiration Induced by Glucose Stimulation and Variation of Adenylate Energy Charge in Glucose-Starved Cells of Green Alga Chlorella Protothecoides  
Authors: Qingyu, Wu
Grant, Neil
Keywords: alternative respiration; adenylate energy charge; glucose; Chlorella protothecoides  
Issue Date: 31-Dec-2001
Publisher: Tsinghua University, China
Citation: Tsinghua Science and Technology (ISSN: 1007-0212) Vol 6 Num 3
Abstract: Effects of inhibitors and glucose on cytochrome and alternative respiration and on adenylate energy charge (AEC) in glucose-starved Chlorella protothecoides   were investigated. 1 mmol/L azide (NaN3), which immediately caused an increase of O2 uptake by inhibiting the cytochrome pathway and stimulating alternative respiration, resulted in a decrease of AEC value from 0.83 to 0.34 within 3 minutes. When 1 mmol/L salicylhydroxamic acid (SHAM) was added into the cell suspension, there was no apparent variation in AEC. Adding NaN3 and SHAM together into cell suspension to inhibit both cytochrome and alternative pathways showed a same change of AEC as that of adding NaN3 alone. When 2.0 mmol/L of glucose was added to a suspension of glucose-starved cells, the O2 uptake rate was immediately stimulated from 0.81 up to 1.34 ] mmol/L O2 ·min-1 ·(mL PCV)-1 ] . The respiration stimulated by glucose could be inhibited about 20% by adding 1 mmol/L SHAM. It was found by titration with SHAM in the absence and presence of NaN3 that 53% of O2 uptake went through the cytochrome pathway and 45% of the alternate pathway was operational in enhanced respiration. It implied that induced operation of the alternative respiratory pathway probably resulted from the burst of the electron flux into the electron transport chain by glucose stimulation.
URI: http://hdl.handle.net/1807/22040
Other Identifiers: http://www.bioline.org.br/abstract?id=ts01072
Rights: Copyright 2001 - Tsinghua Science and Technology
Appears in Collections:Bioline International Legacy Collection

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