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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/22794


Title: Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
Authors: Matos, Denise Cristina Souza
Marcovistz, Rugimar
Cabello, Pedro Hernan
Georgini, Ricardo Amaral
Sakauchi, Dirce
Leite da Silva, Luciana
Keywords: diphtheria-tetanus vaccine - diphtheria-tetanus-pertussis vaccine - toxin binding inhibition test - tetanus - quality control - seroneutralization
Issue Date: 31-Dec-2002
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 97 Num 6
Abstract: Samples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanus-pertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement and a reduction in use of animals can be achieved.
URI: http://hdl.handle.net/1807/22794
Other Identifiers: http://www.bioline.org.br/abstract?id=oc02175
Rights: Copyright 2002 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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