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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/23099


Title: Identification of species related to Anopheles (Nyssorhynchus)albitarsis by random amplified polymorphic DNA-polymerase chain reaction (Diptera: Culicidae)
Authors: Wilkerson, Richard C.
Gaffigan, Thomas V.
Lima, José Bento
Keywords: Anopheles (Nyssorhynchus) albitarsis -Anopheles (Nyssorhynchus) marajoara - Anopheles (Nyssorhynchus) deaneaorum - RAPD PCR - identification
Issue Date: 31-Dec-1995
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 90 Num 6
Abstract: Species-specific Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) markers were used to identify four species related to Anopheles (Nyssorhynchus) albitarsis Lynch-Arribalphalzaga from 12 sites in Brazil and 4 in Venezuela. In a previous study (Wilkerson et al. 1995), which included sites in Paraguay and Argentina, these four species were designated "A", "B", "C" and "D". It was hypothesized that species A is An. (Nys.) albitarsis, species B is undescribed, species C is An. (Nys.) marajoara Galvpio and Damasceno and species D is An. (Nys.) deaneorum Rosa-Freitas. Species D, previously characterized by RAPD-PCR from a small sample from northern Argentina and southern Brazil, is reported here from the type locality of An. (Nys.) deaneorum, Guajarbeta-Mirim, State of Rondnia, Brazil. Species C and D were found by RAPD-PCR to be sympatric at Costa Marques, State of Rondonia, Brazil. Species A and C have yet to be encountered at the same locality. The RAPD markers for species C were found to be conserved over 4,620 km; from Iguape, State of Spio Paulo, Brazil to Rio Socuavo, State of Zulia, Venezuela. RAPD-PCR was determined to be an effective means for the identification of unknown specimens within this species complex.
URI: http://hdl.handle.net/1807/23099
Other Identifiers: http://www.bioline.org.br/abstract?id=oc95147
Rights: Copyright 1995 Fundacao Oswaldo Cruz, FIOCRUZ
Appears in Collections:Bioline International Legacy Collection

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