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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/23458


Title: Quantitation of HIV-1 RNA viral load using NASBA methodology and comparison with other surrogate markers for disease progression
Authors: Sitnik, Roberta
Pinho, Joao Renato Rebello
Keywords: Human Immunodeficiency Virus type 1 - viral load - Nucleic Acid Sequence Based Amplification (NASBA) - surrogate markers
Issue Date: 31-Dec-1998
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 93 Num 3
Abstract: In this study, HIV-1 viral load quantitation determined by Nucleic Acid Sequence Based Amplification (NASBA) was compared with other surrogate disease progression markers (antigen p24, CD4/CD8 cell counts and b-2 microglobulin) in 540 patients followed up at Sao Paulo, SP, Brazil. HIV-1 RNA detection was statistically associated with the presence of antigen p24, but the viral RNA was also detected in 68% of the antigen p24 negative samples, confirming that NASBA is much more sensitive than the determination of antigen p24. Regarding other surrogate markers, no statistically significant association with the detection of viral RNA was found. The reproducibility of this viral load assay was assessed by 14 runs of the same sample, using different reagents batches. Viral load values in this sample ranged from 5.83 to 6.27 log (CV = 36 %), less than the range (0.5 log) established to the determination of significant viral load changes.
URI: http://hdl.handle.net/1807/23458
Other Identifiers: http://www.bioline.org.br/abstract?id=oc98080
Rights: Copyright 1998 Fundacao Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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