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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/23568


Title: Expression of recombinant antigens in Escherichia coli: application on immunochemical studies of Schistosoma mansoni tegumental antigens
Issue Date: 31-Dec-1997
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 92 Num 5
Abstract: Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 92(5), September/October 1997, pp. 637-641 Expression of recombinant antigens in Escherichia coli: application on immunochemical studies of Schistosoma mansoni tegumental antigens Frederico GC Abath, Edeneide M Xavier, Joanne D'Arc B Silva; Marcos A Morais Junior, Silvia ML Montenegro Code Number:OC97119 Sizes of Files: Text: 17.5K Graphics: Tables (jpg) - 65K Line drawings and photographs (jpg) - 23.6K Sm15 and Sm13 are recognized by antibodies from mice protectively vaccinated with tegumental membranes, suggesting a potential role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMal vectors so that comparisons could be made among different expression systems and different genes. The fusion proteins corresponding to several parts of the gene for the precursor of Sm15 failed in producing antibodies recognizing the parasite counterpart. On the other hand, antibodies raised against Sm13 MBP-fusion proteins recognized the 13 kDa tegumental protein. Thus the peculiarities of the gene of interest are important and the choice of the expression system must sometimes be decided on an empirical basis. Key words: Schistosoma mansoni - tegumental antigens - fusion proteins Copyright 1997 Fundacao Oswaldo Cruz
URI: http://hdl.handle.net/1807/23568
Other Identifiers: http://www.bioline.org.br/abstract?id=oc97119
Rights: Copyright 1997 Fundação Oswaldo Cruz, Fiocruz
Appears in Collections:Bioline International Legacy Collection

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