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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/23624


Title: A heminested polymerase chain reaction for the detection of Brazilian rabies isolates from vampire bats and herbivores
Authors: Soares, R. M.
Bernardi, F.
Sakamoto, S. M.
Heinemann, M. B.
Cortez, A.
Alves, L. M.
Meyer, A. D.
Ito, F. H.
Richtzenhain, L. J.
Keywords: rabies virus, polymerase chain reaction, detection of viral RNA, rabies diagnosis, Brazil
Issue Date: 31-Dec-2002
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 97 Num 1
Abstract: A heminested-PCR (hn-PCR) using primers to the nucleoprotein-coding gene in a nested set was evaluated in the detection of Brazilian strains of rabies virus (RV). A representative number of RV nucleoprotein sequences belonging to genotype 1 were aligned. Based on such alignment, primers were directed to highly conserved regions. All 42 clinical samples positive by both fluorescent antibody and mouse inoculation tests were also positive by the hn-PCR. Brain tissue that had been left to decompose, obtained from an experimentally inoculated mouse was tested by hn-PCR and yielded positive results. In conclusion, primers designed here were capable of amplifying Brazilian RV isolates obtained from a rural epidemiological cycle.
URI: http://hdl.handle.net/1807/23624
Other Identifiers: http://www.bioline.org.br/abstract?id=oc02020
Rights: Copyright 2002 Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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