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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/23638


Title: Expression of the major surface antigen of Toxoplasma gondii in the muscle of balb/c mice
Issue Date: 31-Dec-1998
Publisher: Elfos Scientiae
Citation: Biotecnologia Aplicada (ISSN: 0684-4551) Vol 15 Num 3
Abstract: Biotecnología Aplicada 1998;15:159-161 Expression of the major surface antigen of Toxoplasma gondii in the muscle of balb/c mice Alberto Fachado, Jorge L González, Carlos Hidalgo, Dinorah Torres, Teresita Vidal, Lisset Fonseca, Esteban Alberti, Ana M Montalvo, Léster Leal, Marcelo Nazábal, Regina Amendoeira and Armando Acosta Code Number: BA98024 Size of Files: Text: 19K Graphics: Photographs (jpg) - 53K ABSTRACT The gene, corresponding to the mature protein, of the major surface trophozoites antigen of Toxoplasma gondii (P30) was cloned in the pcDNA3 plasmid under the cytomegalovirus intermediate early promoter and used for the intramuscular immunization of Balb/c mice. The expression of the P30 antigen in the muscle of immunized animals was evaluated by indirect immunofluorescence. The expression of P30 antigen in the muscle of immunized animals was detected three days after immunization whereas in animals immunized with pcDNA3 plasmid and in non-immunized animals the results were negative. Key words: Toxoplasma gondii , P30 antigen, DNA, immunization RESUMEN El gen del principal antígeno de superficie del trofozoito de Toxoplasma gondii (P30) fue clonado en el plásmido pcDNA3 bajo el promotor temprano de citomegalovirus y usado para la inmunización intramuscular de ratones Balb/c. La expresión del antígeno P30 en el músculo de los animales inmunizados fue evaluada tres días después por inmunofluorescencia indirecta. Se detectó la presencia del antígeno en los animales inmunizados, no así en los otros grupos. Palabras claves: Toxoplasma gondii, antígeno P30, ADN, inmunización Copyright 1998 Elfos Scientiae
URI: http://hdl.handle.net/1807/23638
Other Identifiers: http://www.bioline.org.br/abstract?id=ba98024
Rights: Copyright 1998 Elfos Scientiae
Appears in Collections:Bioline International Legacy Collection

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