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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/24098

Title: Acute Inflammatory Response in the Stomach of BALB/c Mice Challenged with Coccoidal Helicobacter pylori  
Authors: Rabelo-Gonçalves, E. M. A.
Nishimura, N. F.
Zeitune, J. M. R.
Keywords: Helicobacter pylori   - morphology - animal model
Issue Date: 31-Dec-2002
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 97 Num 8
Abstract: An experimental murine model was used to verify the viability and pathogenicity of coccoid Helicobacter pylori   . For this purpose, 27 BALB/c mice were inoculated intragastrically with 1 ml broth culture (108organisms/ml) of a coccoid H. pylori clinical isolate. The animals were divided into two groups. Nine were infected on a one-time basis (GA1) and 18 were infected on two consecutive days (GA2). Other 27 mice were inoculated with Brucella broth and divided in the same way; they composed the control group. Mice were killed at 2, 3, 7, 14 and 21 days post inoculation (pi). Fragments of stomach and duodenum were collected, fixed with 12% formalin and stained by hematoxilin-eosin and Giemsa for histopathological examination. Until the 14th day, only reinfected mice had mild-to-moderate inflammatory infiltrate in the stomach. The infiltration was predominantly lymphomonocytic, although plasma cells and eosinophils could be seen. However, at 21st day, severe eosinophilic infiltration was present in the lamina propria and submucosa of gastric corpus. In subgroup GA1, animals presented lymphomonocytic infiltration in the stomach from 14th day pi. Our results showed that coccoid H. pylori was able to induce an acute inflammatory response in stomach of reinfected mice since the initial periods of infection.
URI: http://hdl.handle.net/1807/24098
Other Identifiers: http://www.bioline.org.br/abstract?id=oc02258
Rights: Copyright 2002 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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